Molecular Formula | H2O4S |
Water Solubility | It is sparingly soluble in water. |
Solubility | H2O: 10mg/mL |
Appearance | amorphous powder |
Color | white |
Storage Condition | -20°C |
Sensitive | Hygroscopic |
Physical and Chemical Properties | This product is white or white powder, slightly soluble in water, insoluble in ethanol and ether, heating does not solidify, dilute ammonia water can make it precipitate. This product is a strong basic protein with a strong positive charge. It can quickly bind to the acidic groups necessary to maintain anticoagulant activity in the heparin molecule, so that heparin loses anticoagulant activity. |
Safety Description | S22 - Do not breathe dust. S24/25 - Avoid contact with skin and eyes. |
WGK Germany | 3 |
RTECS | VO9470000 |
FLUKA BRAND F CODES | 21-10 |
TSCA | Yes |
HS Code | 35040090 |
EPA chemical information | Information provided by: ofmpub.epa.gov (external link) |
use | the scientific name of protocatechuic aldehyde is 3,4-dihydroxybenzaldehyde (3,4-dihydroxybenzaldehyde), which is one of the main products of the degradation of salvianolic acid B, a water-soluble component of Salvia miltiorrhiza, and is often used as a quality test index to control the quality of Salvia miltiorrhiza related preparations. Procatechuic aldehyde is the main active ingredient in Sijiqing to increase coronary flow. The effect of dilating coronary artery is the strongest among the components in Sijiqing, and has no obvious effect on myocardial oxygen consumption. Protocatechuic aldehyde can significantly increase the microcirculation perfusion flow in rats with microcirculation disorders. The peak time of action is about 20min after administration, and the action time is short. At the same time, protocatechuic aldehyde can reduce the number of normal red blood cells to spiny meridian cells, and reduce the abnormal morphology of spiny meridian cells, so that the degree of cell aggregation is significantly reduced, and the formation of white blood cell pseudopodia and platelet aggregation are less. Anti-heparin drugs. Used for bleeding caused by overdose of heparin injection. Major operations such as cardiac arteries, heparin-like anticoagulants in blood circulation, and a large amount of heparin anticoagulation during hemodialysis can be neutralized with this product as needed. Adverse reactions and contraindications: bradycardia, chest tightness, dyspnea, lower blood pressure, mostly caused by excessive injection, but also caused by pulmonary hypertension or hypertension. After injection, there may be nausea and vomiting, red and hot flashes, fatigue, etc. The protocatechuic aldehyde can only be applied after purification, and generally has no antigenicity. But very few have allergic reactions, such as urticaria, local pain, angioedema, etc., which generally occur after the second administration. This product can aggravate the thrombocytopenia caused by cardiopulmonary bypass in cardiac surgery. This product has a strong basic group, which can be combined with strong acidic heparin in the body to form a stable complex, so that heparin loses its anticoagulant ability. It still has mild antithrombin prokinase effect, and is generally not used clinically to combat non-heparin-induced anticoagulation. Because this product can combine with some proteins and peptides, it can be used to form complexes with insulin, corticosteroids, etc. to prepare long-acting injections. |
myocardial cell protection | protocatechuic aldehyde can reduce calcium overload and protect myocardium. it has been proved that myocardial cell damage caused by myocardial ischemia and ischemia reperfusion injury is related to intracellular Ca 2 + overload. Electrophysiological studies of myocardial cells show that anaerobic metabolism in cells after ischemia leads to intracellular Na + overload caused by decrease of intracellular pH, and further Ca 2 + overload plays an important role in myocardial ischemia and reperfusion injury. The effects of salvia miltiorrhiza injection and its active components, danshensu and PCA, on the cytoplasmic Ca 2 + concentration of healthy adult erythrocytes in vitro were determined by fluorescence probe Fura-2 quantitative analysis, and the results showed that both danshensu and protocatechualdehyde (PCA) had the effect of reducing the cytoplasmic Ca 2 + concentration of adult erythrocytes in a dose-dependent manner, and the effect was similar to that of salvia miltiorrhiza injection; moreover, the calcium antagonism of PCA is stronger than that of danshensu, and the maximum inhibition rate of erythrocyte cytoplasmic Ca 2 + concentration can reach more than 80%, and there is no bell curve characteristic. PCA has the core structure of o-diphenol, which is the material basis of its significant antioxidant activity. The correlation analysis between the composition combination of Salvia miltiorrhiza and antioxidant activity showed that the antioxidant activity of PCA in scavenging 1, 1-diphenyl-picrhydrazinyl (DPPH) free radicals was stronger than that of sodium danshensu and caffeic acid. Using the in vitro antioxidant model, the in vitro antioxidant capacity of danshensu, PCA, caffeic acid and salvianolic acid B, the representative components of salvia miltiorrhiza medicinal materials, was quantitatively compared. the order of IC 50 values for the removal of OH by the four phenolic compounds was: danshensu> caffeic acid> PCA> salvianolic acid B, indicating that salvianolic acid B had the strongest effect, and the effect of PCA was secondary to salvianolic acid B, these components may be important active components of Salvia miltiorrhiza in the treatment of cardiovascular diseases by scavenging oxygen free radicals. |
production method | 50kg of salvia miltiorrhiza was extracted with 0.4% sodium hydroxide 1200L solution to obtain an extract containing protocatechualdehyde; The extract is adjusted to neutral with hydrochloric acid, adsorbed with an adsorption column filled with weakly alkaline macroporous adsorption resin D301M, and eluted with 50%(V/V)400L of ethanol solution after the adsorbent is saturated, elute with 0.4%(m/V)500L of sodium hydroxide aqueous solution, collect eluent, concentrate to about 20L, adjust to PH value of 2 with hydrochloric acid, carry out acidic conditions (pretreatment, elute treated resin with hydrochloric acid solution until PH value of eluent is 2) with adsorption column filled with weakly polar adsorbent AB-8, elute with 800L of pure water, and then use 5% ethanol solution to elute 800L to collect eluent, concentrate to obtain 2L of concentrated solution; Cooling, standing, obtaining protocatechuic aldehyde crystal, filtering, washing, drying, obtaining protocathuic aldehyde crystal 238g, the purity reached 93% by HPLC detection. |